ccl3  (Boster Bio)

Journal: iScience

Article Title: Blocking CCL3-mediated neutrophil recruitment into the brain alleviates immunopathology following severe enterovirus 71 infection

doi: 10.1016/j.isci.2024.111388

Figure Lengend Snippet:

Article Snippet: human CCL3 ELISA kit , Boster Biological Engineering Co.,Ltd , EK0448.

Techniques: Virus, Recombinant, Enzyme-linked Immunosorbent Assay, Isolation, Knock-Out, Software

Journal: iScience

Article Title: Blocking CCL3-mediated neutrophil recruitment into the brain alleviates immunopathology following severe enterovirus 71 infection

doi: 10.1016/j.isci.2024.111388

Figure Lengend Snippet: CCL3 and CCL2 showed the most robust increases in CSF of patients and mice during severe EV71 infection (A) Density distribution of 27 cytokines fold change of control (red), mild (blue) or critical (green)/control, in the sera of EV71-infected patients. (B) QPCR analysis of cytokines fold changes of critical/recovered CSF of patients. (C and D) CCL3 (C) and CCL2 (D) protein levels in CSF of patients with EV71 and brainstem encephalitis (severe immunopathology) at either the acute phase or recovery phase, measured by ELISA. (E and F) CCL3 (E) and CCL2 (F) protein levels in brain tissues of EV71-infected mice, measured by ELISA. Data are representative of three independent experiments. Data are reported as the mean ± SD, n = 10. ∗ p < 0.05 and ∗∗ p < 0.01 vs isotype antibody or WT mice (unpaired, two-tailed Student’s t test).

Article Snippet: human CCL3 ELISA kit , Boster Biological Engineering Co.,Ltd , EK0448.

Techniques: Infection, Control, Enzyme-linked Immunosorbent Assay, Two Tailed Test

Journal: iScience

Article Title: Blocking CCL3-mediated neutrophil recruitment into the brain alleviates immunopathology following severe enterovirus 71 infection

doi: 10.1016/j.isci.2024.111388

Figure Lengend Snippet: The deficiency of CCL3 reduces neutrophils infiltration in brain and mortality of severe EV71-infected mice 2 weeks old WT, Ccl3 −/− and Ccl2 −/− C57BL/6 mice were injected i.p. with 100LD50/100 μL/mouse of EV71. (A and B) Leukocytes were isolated from brain tissues by Ficoll Reagent and stained with anti-CD45-FITC and anti-Ly6G-PE on day 6 dpi. The percentages of neutrophils (CD45 + Ly6G + ) in total leukocytes were detected by FCM assay (A) and the corresponding statistical analyses are shown (B). (C and D) Immunofluorescence histochemistry analysis Ly6G for neutrophils in brain tissues (C) and the mean fluorescence intensity (MFI) (D) on day 6 dpi. (E and F) HE staining (E) and pathological scores of brain tissues (F) on day 6 dpi. (G–I) The mortality (G), body weight (H) and clinical scores (I) of mice (A) were monitored and recorded daily after infection. Data are representative of three independent experiments. Data are reported as the mean ± SD, n = 10. ∗ p < 0.05 and ∗∗ p < 0.01 vs isotype antibody or WT mice (unpaired, two-tailed Student’s t test).

Article Snippet: human CCL3 ELISA kit , Boster Biological Engineering Co.,Ltd , EK0448.

Techniques: Infection, Injection, Isolation, Staining, Immunofluorescence, Fluorescence, Two Tailed Test

Journal: iScience

Article Title: Blocking CCL3-mediated neutrophil recruitment into the brain alleviates immunopathology following severe enterovirus 71 infection

doi: 10.1016/j.isci.2024.111388

Figure Lengend Snippet: CCL3 recruit neutrophils into the brain through CCR1/3(human) and CCR5(mouse) (A) Transwell assay for chemotactic ability of neutrophils from EV71-infected mice or PBS-injected mice, in which the CCL3 and CCL2 proteins were placed in the lower chamber and the neutrophils were seeded in the upper chamber and 3 h later the neutrophils that had transversed into the lower chamber were counted. Positive control: fMLP; negative control: RPMI-160 medium. (B) qPCR analysis chemokine receptors expression in neutrophils from control, mild and critical EV71 infection patients. (C and E) Expression of CCR1 (C), CCR3 (D) and CCR5 (E) on neutrophils in human CSF from patients with EV71 infection during either the acute phase or recovery phase, measured by qRT-PCR. (F–H) Expression of Ccr1 (F), Ccr3 (G) and Ccr5 (H) on neutrophils in brain tissues from EV71-infected mice or PBS-injected negative control mice, measured by qRT-PCR.

Article Snippet: human CCL3 ELISA kit , Boster Biological Engineering Co.,Ltd , EK0448.

Techniques: Transwell Assay, Infection, Injection, Positive Control, Negative Control, Expressing, Control, Quantitative RT-PCR

Journal: iScience

Article Title: Blocking CCL3-mediated neutrophil recruitment into the brain alleviates immunopathology following severe enterovirus 71 infection

doi: 10.1016/j.isci.2024.111388

Figure Lengend Snippet: Inhibition of CCL3 reduces the neutrophils infiltration in brains and the mortality of EV71 infected mice (A–F) EV71-infected mice (100LD 50 /100 μL/mouse of EV71) were injected i.p. with isotype or anti-CCL3 antibody (100 μg/100 μL/mouse). (A) ELISA analysis the CCL3 expression in mice brain tissues. (C and B) Leukocytes were isolated from brain tissues of EV71-infected mice by use of the Ficoll Reagent on day 6 dpi and stained with anti-CD45-FITC and anti-Ly6G-PE. The percentages of neutrophils (CD45+Ly6G+) in total leukocytes were detected by FCM assay (C) and the corresponding statistical analyses are shown (B). (D–F) The mortality (D), body weight (E) and clinical scores (F) of mice were monitored and recorded daily after infection. (G–L) EV71-infected mice (100LD 50 /100 μL/mouse of EV71) were injected i.p. with Mixed-scrables or Mixed-mmu-mirs (90 μg/g body weight), (G) qPCR analysis the Ccl3 mRNA expression in mice brain tissues. (H and I) Leukocytes were isolated from brain tissues of EV71-infected mice by use of the Ficol Reagent on day 6 dpi and stained with anti-CD45-FITC and anti-Ly6G-PE. The percentages of neutrophils (CD45 + Ly6G + ) in total leukocytes were detected by FCM assay (H) and the corresponding statistical analyses are shown (I). (J–L) The mortality (J), body weight (K) and clinical scores (L) of mice (G) were monitored and recorded daily after infection.

Article Snippet: human CCL3 ELISA kit , Boster Biological Engineering Co.,Ltd , EK0448.

Techniques: Inhibition, Infection, Injection, Enzyme-linked Immunosorbent Assay, Expressing, Isolation, Staining

Journal: iScience

Article Title: Blocking CCL3-mediated neutrophil recruitment into the brain alleviates immunopathology following severe enterovirus 71 infection

doi: 10.1016/j.isci.2024.111388

Figure Lengend Snippet:

Article Snippet: human CCL3 ELISA kit , Boster Biological Engineering Co.,Ltd , EK0448.

Techniques: Virus, Recombinant, Enzyme-linked Immunosorbent Assay, Isolation, Knock-Out, Software